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Journal of Agricultural Science and Technology B2 (2012) 988-996, ISSN 1939-1250

Response of Juvenile Freshwater Prawn Macrobrachium rosenbergii (de Man) to Different Levels of Bacillus subtilis Isolated from Chicken Intestine as Probiotics

M.A. Keysami1, M. Mohammadpour2

1. Department of Aquaculture, Persian Gulf Jihad Agriculture Technical and Vocational Higher education Center, Bushehr3588, Iran

2. Department of Research Management, Bushehr University of Medical Sciences, Bushehr, Iran

 

Received: June 11, 2012 /Published: September 20, 2012.

 

Abstract: A feeding experiment was conducted to investigate the effect of Bacillus subtilisbacterium and its suitable levelon survival and growth rate of juvenile Macrobrachium rosenbergii (de Man) during on 28 March to 28 May, 2010. AB. subtilis bacterium isolated from chicken intestine was added to commercial prawn feed as a probiotic. Six diets were prepared by soaking prawn feed in to the B. subtilis to achieve 1010, 109, 108, 107 and 106 levels with a non-treated control. After 60 days, the prawns fed diet at level 108 showed a higher mean weight gain (593.45 g) or 170.29% increase in growth over control. The mean weight gain showed a decreasing trend as the B. subtilis level decreased from 108 to 106 and the B. subtilis level increased from 108 to 1010 in the diets. There were significant differences (P<0.05) in weight gain, feed intake, daily growth and feed conversion ratio (FCR) among treated and controlled groups. There were significant differences (P<0.05) among treatments and control in survival rate but no significant differences (P>0.05) in water quality and biochemical composition among treated and controlled groups. Clearly, treated with B. subtilis appeared to enhance growth and survival of M. rosenbergii. It was concluded that the tested strain may be a promising probiotics for M. rosenbergii at a level of 108 B. subtilisin to the prawn feed.

 

Key words: Bacillus subtilis, Macrobrachium rosenbergii, level of probiotics.